Southern, Northern, and Western Blot: What’s The Difference? The main difference between Southern, northern, and western blot techniques is the target molecules. While western blots target proteins in ...
The Western blot workflow for immuno-based protein detection was introduced over 30 years ago. Since its introduction, Western blotting has become a well-known and widely accepted technique routinely ...
Discover how decisions about experimental design can affect Western blotting reproducibility – and how to make the right decisions in this webinar from Sally Mulford, Sr Molecular Biologist at LI-COR ...
This article describes the histone western blot protocol, which is often used at Abcam for identifying histone proteins obtained from purified calf thymus. For each lane, either 0.5 μg of calf thymus ...
Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...
Western blots have been a tried-and-true method for separating and quantifying proteins based on molecular weight, but they have not kept up with the continually increasing output demands of modern ...
Bio-Rad Laboratories, Inc. have released the first set in a new product line of PrecisonAbTM Antibodies, rigorously validated for use in western blotting. These antibodies offer superior sensitivity, ...
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